LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1616..1712) /label=attL2 /note="attL2 (c)" misc_signal complement(1727..1746) /note="T7 Promoter/priming site (c)" primer_bind 1754..1770 /note="M13 Reverse priming site" gene 1883..2692 /note="Kanamycin resistance gene" rep_origin 2813..3486 /note="pUC origin" vector join(1624..3489,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1609 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:61459 IMAGE:5752337" /tissue_type="Brain, Lung, Testis, adult, pooled whole" /clone_lib="NIH_MGC_115" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1609 /gene="ST8SIA4" /gene_synonym=MGC34450 /gene_synonym=MGC61459 /gene_synonym=PST /gene_synonym=PST1 /gene_synonym=ST8SIA-IV /db_xref="GeneID:7903" /db_xref="HGNC:10871" /db_xref="MIM:602547" CDS 692..1609 /dnas_title="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4" /gene="ST8SIA4" /gene_synonym=MGC34450 /gene_synonym=MGC61459 /gene_synonym=PST /gene_synonym=PST1 /gene_synonym=ST8SIA-IV /codon_start=1 /product="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4" /protein_id="AAH53657.1" /db_xref="GI:31566341" /db_xref="GeneID:7903" /db_xref="HGNC:10871" /db_xref="MIM:602547" /translation="MRSIRKRWTICTISLLLIFYKTKEIARTEEHQETQLIGDGELSL SRSLVNSSDKIIRKAGSSIFQHNVEGWKINSSLVLEIRKNILRFLDAERDVSVVKSSF KPGDVIHYVLDRRRTLNISHDLHSLLPEVSPMKNRRFKTCAVVGNSGILLDSECGKEI DSHNFVIRCNLAPVVEFAADVGTKSDFITMNPSVVQRAFGGFRNESDREKFVHRLSML NDSVLWIPAFMVKGGEKHVEWVNALILKNKLKVRTAYPSLRLIHAVRGYWLTNKVPIK RPSTGLLMYTLATRFCDEIHLYGFWPFPKDLNGKAVKYHYYDDLKYRYFSNASPHRMP LEFKTLNVLHNRGALKLTTGKCVKQ" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaaatcatt cgaaaggctg gctcttcaat 721 cttccagcac aatgtagaag gttggaaaat caattcctct ttggtcctag agataaggaa 781 gaacatactt cgtttcttag atgcagaacg agatgtgtca gtggtcaaga gcagttttaa 841 gcctggtgat gtcatacact atgtgcttga caggcgccgg acactaaaca tttctcatga 901 tctacatagc ctcctacctg aagtttcacc aatgaagaat cgcaggttta agacctgtgc 961 agttgttgga aattctggca ttctgttaga cagtgaatgt ggaaaggaga ttgacagtca 1021 caattttgta ataaggtgta atctagctcc tgtggtggag tttgctgcag atgtgggaac 1081 taaatcagat tttattacca tgaatccatc agttgtacaa agagcatttg gaggctttcg 1141 aaatgagagt gacagagaaa aatttgtgca tagactttcc atgctgaatg acagtgtcct 1201 ttggattcct gctttcatgg tcaaaggagg agagaagcac gtggagtggg ttaatgcatt 1261 aatccttaag aataaactga aagtgcgaac tgcctatccg tcattgagac ttattcatgc 1321 tgtcagaggt tactggctga ccaacaaagt tcctatcaaa agacccagca caggtcttct 1381 catgtataca cttgccacaa gattctgtga tgaaattcac ctgtatggat tctggccctt 1441 ccctaaggat ttaaatggaa aagcggtcaa atatcattat tatgatgact taaaatatag 1501 gtacttttcc aatgcaagcc ctcacagaat gccattagaa ttcaaaacat taaatgtgct 1561 acataataga ggagctctaa aactgacaac aggaaagtgt gtaaagcaaT AGGACCCAGC 1621 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 1681 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 1741 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 1801 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 1861 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 1921 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 1981 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2041 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2101 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2161 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2221 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2281 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2341 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 2401 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 2461 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 2521 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 2581 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 2641 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 2701 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 2761 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 2821 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 2881 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 2941 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3001 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3061 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3121 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3181 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3241 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3301 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3361 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 3421 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 3481 TCACATGTT //